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當(dāng)前位置:首頁   >  產(chǎn)品中心  >    >  植物  >  CCM-300葉綠素含量測量儀

葉綠素含量測量儀

簡要描述:CCM-300葉綠素含量測量儀是使用葉綠素?zé)晒獗嚷蕦θ~綠素含量測量的一種新型儀器。與傳統(tǒng)的化學(xué)測量方法和吸收性測量方法相比,它可以對非常小的葉片以及難以測量樣品的葉綠素含量進行非破壞性測量,測量迅速可靠。

  • 產(chǎn)品型號:CCM-300
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-10-15
  • 訪  問  量:1164

詳細介紹

    一用途

    葉綠素含量測量儀廣泛應(yīng)用于植物生理生態(tài)研究,特別是對傳統(tǒng)吸收法測量困難的小葉片或樣品的葉綠素含量進行測量,例如針葉、發(fā)育未*的水稻、生于巖石上的絲狀藻、地衣、草坪草、仙人掌、龍舌蘭屬植物、菠蘿、擬南芥、果實、苔蘚、葉莖、葉柄。

 

0002.png

 

    二原理

    盡管實踐已經(jīng)證明了吸收技術(shù)測量葉綠素含量的有效性,但該方法對樣品的要求較高,使其應(yīng)用范圍受到限制。而樣品本身的葉綠素?zé)晒鈩t不受樣品厚度、均一性等的影響,可以指示葉綠素的含量。根據(jù)這個原理,Gitelson等比較了葉片的吸收和反射對葉綠素?zé)晒獾挠绊?,發(fā)現(xiàn)在735nm和700nm處的比值與葉片的反射相關(guān)性最高,并且當(dāng)樣品葉綠素含量在41mg·m-2到675mg·m-2時,F(xiàn)735/F700的比值與其葉綠素含量間存在著顯著的線性關(guān)系(R2≥0.95),與傳統(tǒng)的吸收性測量方法相比(測量范圍≤300mg·m-2),提高了葉綠素含量的線性關(guān)系范圍。

    根據(jù)Gitelson的研究結(jié)果,CCM-300使用具有15nm半峰寬的460nm二極管用于熒光激發(fā),提高了紅色熒光波長,避開了大約685nm的發(fā)射熒光峰(葉綠素可重新吸收,降低測量精度)。儀器使用在735nm和700nm的葉綠素發(fā)射熒光的比率(F735/F700)進行葉綠素含量的測量,讀數(shù)為F735/F700的比值以及根據(jù)Gitelson方程計算的葉綠素含量。

 

三 特點

 

Ø 直接算出葉綠素含量mg/m2;

 

Ø 測量結(jié)果可靠,不受葉片或樣品大小、厚度和形狀的影響;

 

Ø 經(jīng)濟、輕便、手持式設(shè)計,適合野外使用;

 

Ø 與化學(xué)測量結(jié)果高度相關(guān),即使在高葉綠素含量時;

 

Ø 彩色觸摸屏顯示;

 

Ø 離散的單點測量和多點(2-30個樣品)平均值測量模式;

 

Ø 非易失性閃存(2GB);

 

USB數(shù)據(jù)輸出,可使用Excel打開;

 

0001.png

 

 

 

四 組成

 

主機、光纖樣品夾、電池充電器、4節(jié)AA鎳氫充電電池、USB通訊纜線使用手冊。

 

可選配件:

 

20mm大探頭: 增加測量面積。 較大的樣本量可能對某些類型的樣本有用,例如苔蘚

 

 

0004.png

 

 

0005.png

 

 

 

增長版葉夾:

 

0006.png

 

 

0007.png

 

 

 

 



技術(shù)參數(shù)

 


 

 

Ø 測量參數(shù):葉綠素?zé)晒獗嚷?/span>——發(fā)射熒光在735nm/700nm的比值(CFR),或相對葉綠素含量mg· m-2

 

Ø 測量面積:直徑3mm的圓,外徑4mm,但對直徑小于3mm的樣品也可準確可靠的測量;

 

Ø 分辨率:比值0.011 mg· m-2;

 

Ø 重復(fù)性:依賴于信號強度,對信號強度低的樣品,建議使用多點測量的平均值,對信號強度高的樣品,差異通常為±0.03或更低;。

 

Ø 噪聲:<±0.02;

 

Ø 光源:LED 460 nm藍光二極管,半峰寬15 nm;

 

Ø 探頭:兩個固態(tài)高感傳感器,提供濾光片。同時提供兩個波長探頭(700nm~710nm730nm~740nm);

 

Ø 檢測:數(shù)控調(diào)制光,將背景光影響將至低。并對光源和檢測器進行溫度補償;

 

Ø 存儲容量:2GB

 

Ø 模式:單點測量,2~30個點去平均值,去除2σ區(qū)間外的平均值,或者中位數(shù);

 

Ø 樣品接口:光纖傳感器,直徑4mm;

 

Ø 用戶界面:240 × 320 像素彩色觸摸屏;

 

Ø 輸出:USB 1.1;

 

Ø 溫度范圍:0~50 ℃;

 

Ø 電源:2節(jié)充電AA電池;

 

Ø 自動關(guān)閉間隔:(無按鍵或下載)0~20分鐘可選;

 

Ø 大?。?/span>12 cm × 9 cm × 3 cm;

 

Ø 重量0.16lbs 275g;

 

Ø 測量時間:5秒;

 


 

 

五、產(chǎn)地:美國

 

六、參考文獻

 

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南極的苔蘚

 

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Casanova-Katny, (2017) Passive warming reduces stress and shifts reproductive effort in the Antarctic moss,

 

Polytrichastrum alpinum Annals of Botany, Volume 119, Issue 1, 1 January 2017, Pages 27–38,

 

 

 

 

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Dana Riddle (2015) Coral Nutrition Part 4: Can Nutrient Deficiency Related to Water Motion Induce Coral

 

Blea

 

葉綠素

 

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Chuan-Sheng Wu (2019) Comparison of two noninvasive methods for measuring the pigment content in foliose

 

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果園草

 

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Cutting Height and High Temperature on Regrowth Vigor in Orchard grass. Front Plant Sci. 2017; 8: 805.

 

Published online 2017 May 19. doi:  10.3389/fpls.2017.00805 PMCID: PMC5437204

 

7-42天的轉(zhuǎn)基因大麥

 

PetrVojta, FilipKokáš, Alexandra Husi?ková, Ji?íGrúz, VeroniqueBergougnoux, Cintia F. Marchetti, Eliška

 

Je?ilová, Václav Mik, Yoshihisa Ikeda, Petr Galuszka, (2016) Whole transcriptome analysis of transgenic

 

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Volume 33, Issue 5, Part B, 25 September 2016, Pages 676-691 

 

蘇格蘭松樹幼苗

 

Tapani Repo, Samuli Launiainen, Tarja Lehto, Sirkka Sutinen, Hanna Ruhanen, Juha Heiskanen, Ari Laurén,

 

Raimo Silvennoinen, Elina Vapaavuori, Leena Finér (2016) The responses of Scots pine seedlings to

 

waterlogging during the growing season   Canadian Journal of Forest Research 2016, 46(12): 1439-1450,

 

 

 

 

挪威云杉和蘇格蘭松樹苗

 

Johanna Riikonen, Nelli Kettunen, Maria Gritsevich, Teemu Hakala, Liisa Särkkä, Risto Tahvonen, (2016)

 

Growth and development of Norway spruce and Scots pine seedlings under different light spectra

 

Environmental and Experimental Botany Volume 121, January 2016, Pages 112-

 

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Anshu Rastogi, Marcin Stró?eckia, Hazem M. Kalaji, Dominika ?uców de Mariusz, Lamentowicz, Rados?aw

 

Juszczak (2019) Impact of warming and reduced precipitation on photosynthetic and remote sensing properties

 

of peatland vegetation Environmental and Experimental Botany Volume 160, April 2019, Pages 71-80

 

CAM植物 - 仙人掌

 

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DACTYLOPIUS OPUNTIAE (COCKERELL) (HEMIPTERA: DACTYLOPIIDAE). University of the

 

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Developmental Traits in Silvery-Thread Moss (Bryum argenteum Hedw.) Genotypes between Golf Course

 

Putting Greens and Native Habitats. Cambridge University Press: 11 September 2018, Volume 66, Issue 5 ,

 

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MJ Clark, Y Zheng (2017) Effect of Top-dressed Controlled-release Fertilizer Rates on Nursery Crop

 

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大豆

 

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States. Science of The Total Environment,–Volumes 593594, 1 September 2017, Pages 263-273

 

 

 

 

土豆

 

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Assessment of Growth, Tuber Elemental Composition, Stomatal Conductance and Chlorophyll Content of Two

 

Potato C*rs Under Irrigation with Fly Ash-Treated Acid Mine Drainage. American Journal of Potato

 

Researc August 2017, Volume 94,  Issue4, pp 367–378

 

十字花科-開花植物

 

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(Brassicaceae) Populations from Non-metalliferous Sites in the Eastern Pyrenees Front Plant Sci. 2016; 7: 128.

 

Published online 2016 Feb 9. doi:10.3389/fpls.2016.00128

 

毒藤

 

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Availability by Geographically Isolated Poison Ivy Accessions. Northeastern Naturalist 24(2):191-200. 2017

 

 

 

 

糖楓

 

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道路旁植物中的鎘和銅脅迫

 

Margita Kuklová, Helena Hnili?ková, František Hnili?ka, Ivica Pivková, Ján Kukla (2019) Impact of

 

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